Composite

Part:BBa_K3352005:Design

Designed by: Hannah Hsu   Group: iGEM20_TAS_Taipei   (2020-10-18)


Strong Promoter and RBS phi29 DNA Polymerase Ligase Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 656
    Illegal SapI.rc site found at 1054


Design Notes

We added a His-Tag to the part so that we could perform a His-purification on the protein.


Source

We obtained the amino acid sequence of phi29 DNA polymerase from Bacillus phage phi29.

References